Comparison of UPLC and HPLC methods for determination of vitamin C
Inga Klimczak , Anna Gliszczyńska-Świgło
AbstractUltra performance liquid chromatography (UPLC) and high-performance liquid chromatography (HPLC) methods for determination of ascorbic acid (AA) and total AA (TAA) contents (as the sum of AA and dehydroascorbic acid (DHAA) after its reduction to AA) in fruit beverages and in pharmaceutical preparations were compared. Both methods are rapid: total time of analysis was 15 and 6 min for HPLC and UPLC methods, respectively. The methods were validated in terms of linearity, instrument precision, limits of detection (LOD) and quantification (LOQ), accuracy and recovery. Intra- and inter-day instrument precisions for fruit juices, expressed as RSD, were 2.2% and 2.4% for HPLC, respectively, and 1.7% and 1.9% for UPLC, respectively. For vitamin C tablets, inter- and intra-day precisions were 0.4% and 0.5%, respectively (HPLC), and 0.5% and 0.3%, respectively (UPLC). Both methods were sensitive: LOD was 0.049 μg/mL for HPLC and 0.024 μg/mL for UPLC while LOQs were 0.149 and 0.073 μg/mL for HPLC and UPLC, respectively. These methods could be useful in the routine qualitative and quantitative analysis of AA or TAA in pharmaceutical preparations or fruit beverages. However, UPLC method is more sensitive, faster and consumes less eluent.
|Journal series||Food Chemistry, ISSN 0308-8146, (A 40 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||HPLC; UPLC; Vitamin C; Food analysis; Beverages; Pharmaceutical preparations|
|ASJC Classification||; ;|
|Score|| = 40.0, 05-02-2020, ArticleFromJournal|
= 40.0, 05-02-2020, ArticleFromJournal
|Publication indicators||= 41; : 2015 = 1.955; : 2015 = 4.052 (2) - 2015=4.232 (5)|
|Citation count*||117 (2020-10-22)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.