Comparison of UPLC and HPLC methods for determination of vitamin C

Inga Klimczak , Anna Gliszczyńska-Świgło


Ultra performance liquid chromatography (UPLC) and high-performance liquid chromatography (HPLC) methods for determination of ascorbic acid (AA) and total AA (TAA) contents (as the sum of AA and dehydroascorbic acid (DHAA) after its reduction to AA) in fruit beverages and in pharmaceutical preparations were compared. Both methods are rapid: total time of analysis was 15 and 6 min for HPLC and UPLC methods, respectively. The methods were validated in terms of linearity, instrument precision, limits of detection (LOD) and quantification (LOQ), accuracy and recovery. Intra- and inter-day instrument precisions for fruit juices, expressed as RSD, were 2.2% and 2.4% for HPLC, respectively, and 1.7% and 1.9% for UPLC, respectively. For vitamin C tablets, inter- and intra-day precisions were 0.4% and 0.5%, respectively (HPLC), and 0.5% and 0.3%, respectively (UPLC). Both methods were sensitive: LOD was 0.049 μg/mL for HPLC and 0.024 μg/mL for UPLC while LOQs were 0.149 and 0.073 μg/mL for HPLC and UPLC, respectively. These methods could be useful in the routine qualitative and quantitative analysis of AA or TAA in pharmaceutical preparations or fruit beverages. However, UPLC method is more sensitive, faster and consumes less eluent.
Author Inga Klimczak (WT / KTAS)
Inga Klimczak,,
- Department of Food Commodity Science
, Anna Gliszczyńska-Świgło (WT / KTiAI)
Anna Gliszczyńska-Świgło,,
- Department of Technology and Instrumental Analysis
Journal seriesFood Chemistry, ISSN 0308-8146, (A 40 pkt)
Issue year2015
Publication size in sheets0.5
Keywords in EnglishHPLC; UPLC; Vitamin C; Food analysis; Beverages; Pharmaceutical preparations
ASJC Classification2700 General Medicine; 1106 Food Science; 1602 Analytical Chemistry
Languageen angielski
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 40.0, 05-02-2020, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 05-02-2020, ArticleFromJournal
Publication indicators WoS Citations = 41; Scopus SNIP (Source Normalised Impact per Paper): 2015 = 1.955; WoS Impact Factor: 2015 = 4.052 (2) - 2015=4.232 (5)
Citation count*117 (2020-10-22)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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