The Genoprotective Role of Naringin

Oskar Szczepaniak , Marta Ligaj , Joanna Kobus-Cisowska , Mariusz Tichoniuk , Marcin Dziedziński , Monika Przeor , Piotr Szulc

Abstract

Since ancient times, fruits and edible plants have played a special role in the human diet for enhancing health and maintaining youthfulness. The aim of our work was to determine the interactions between naringin, a natural ingredient of grapefruits, and DNA using an electrochemical biosensor. Electrochemical methods allow analyzing the damages occurring in the structure of nucleic acids and their interactions with xenobiotics. Our study showed that the changes in the location of electrochemical signals and their intensity resulted from the structural alterations in DNA. The signal of adenine was affected at lower concentrations of naringin, but the signal of guanine was unaffected in the same condition. The dynamics of changes occurring in the peak height and surface of adenine related to naringin concentration was also significantly lower. The complete binding of all adenine bases present in the tested double-stranded DNA solution was observed at naringin concentrations ranging from 8.5 to 10.0 µM. At larger concentrations, this active compound exerted an oxidizing effect on DNA. However, the critical concentrations of naringin were found to be more than twice as high as the dose absorbable in an average human (4 µM). The results of our work might be helpful in the construction of electrochemical sensors for testing the content of polyphenols and would allow determining their genoprotective functionality.
Author Oskar Szczepaniak
Oskar Szczepaniak,,
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, Marta Ligaj (WT / KTiEPP)
Marta Ligaj,,
- Department of Industrial Products and Ecology
, Joanna Kobus-Cisowska
Joanna Kobus-Cisowska,,
-
, Mariusz Tichoniuk (WT / KTiEPP)
Mariusz Tichoniuk,,
- Department of Industrial Products and Ecology
, Marcin Dziedziński
Marcin Dziedziński,,
-
, Monika Przeor
Monika Przeor,,
-
, Piotr Szulc
Piotr Szulc,,
-
Journal seriesBiomolecules, ISSN 2218-273X, e-ISSN 2218-273X, (N/A 100 pkt)
Issue year2020
No10(5)
Pages1-20
Publication size in sheets0.95
Article number700
Keywords in Polishnaringina, DNA, reaktywne formy tlenu, elektrochemiczny biosensor DNA, woltamperometria fali prostokątnej
Keywords in Englishnaringin, DNA, reactive oxygen species, electrochemical DNA biosensor, square-wave voltammetry
ASJC Classification1303 Biochemistry; 1312 Molecular Biology
DOIDOI:10.3390/biom10050700
URL https://www.mdpi.com/2218-273X/10/5/700
Languageen angielski
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 06-05-2020, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.641; WoS Impact Factor: 2018 = 4.694 (2)
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UwagiThis article belongs to the Special Issue Biomolecules in Complex Biological and Biochemical Systems – Selected Papers from ICBB2019
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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