Investigation of the effectiveness of disinfectants against planktonic and biofilm forms of P. aeruginosa and E. faecalis cells using a compilation of cultivation, microscopic and flow cytometric techniques

Wojciech Juzwa , Kamila Myszka , Wojciech Białas , Renata Dobrucka , Piotr Konieczny , Karolina Czaczyk

Abstract

This study evaluated the effectiveness of selected disinfectants against bacterial cells within a biofilm using flow cytometry, the conventional total viable count test and scanning electron microscopy (SEM). A flow cytometric procedure based on measurement of the cellular redox potential (CRP) was demonstrated to have potential for the rapid evaluation of activity against biofilm and planktonic forms of microbes. Quaternary ammonium compound-based disinfectant (QACB) demonstrated a higher level of anti-microbial activity than a performic acid preparation (PAP), with mean CRP values against P. aeruginosa cells of 2 and 1.33 relative fluorescence units (RFU) vs 63.33 and 61.33 RFU for 8 and 24 h cultures respectively. Flow cytometric evaluation of the anti-biofilm activity demonstrated a higher efficacy of QACB compared to PAP for P. aeruginosa cells of 1 and 0.66 RFU vs 18.33 and 22.66 RFU for 8 and 24 h cultures respectively. SEM images of treated P. aeruginosa cells demonstrated disinfectant-specific effects on cell morphology.
Author Wojciech Juzwa - Poznań University of Life Sciences
Wojciech Juzwa,,
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, Kamila Myszka - Uniwersytet Przyrodniczy w Poznaniu
Kamila Myszka,,
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, Wojciech Białas - Uniwersytet Przyrodniczy w Poznaniu
Wojciech Białas,,
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, Renata Dobrucka (WT / KTiEPP)
Renata Dobrucka,,
- Department of Industrial Products and Ecology
, Piotr Konieczny - Uniwersytet Przyrodniczy w Poznaniu
Piotr Konieczny,,
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, Karolina Czaczyk - Uniwersytet Przyrodniczy w Poznaniu
Karolina Czaczyk,,
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Journal seriesBiofouling, ISSN 0892-7014, (A 40 pkt)
Issue year2015
Vol31
No7
Pages587-597
Publication size in sheets0.5
Keywords in Englishbiofilm, germicidal activity, disinfectant, flow cytometric analysis, technological surface
ASJC Classification1104 Aquatic Science; 2402 Applied Microbiology and Biotechnology; 2312 Water Science and Technology
DOIDOI:10.1080/08927014.2015.1075126
URL http://www.tandfonline.com/doi/pdf/10.1080/08927014.2015.1075126
Languageen angielski
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 40.0, 05-02-2020, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 05-02-2020, ArticleFromJournal
Publication indicators WoS Citations = 6; Scopus SNIP (Source Normalised Impact per Paper): 2015 = 1.151; WoS Impact Factor: 2015 = 3.0 (2) - 2015=3.423 (5)
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