Application of multidimensional and conventional fluorescence techniques for classification of beverages originating from various berry fruit

Ewa Sikorska , Katarzyna Włodarska , Igor Khmelinskii


The objectives of this study were to characterize fluorescence of beverages from berry fruit, including chokeberry, blackcurrant, raspberry and strawberry, and to develop classification models based on different types of fluorescence spectra to identify beverages depending on the fruit species. Total fluorescence spectra (excitation-emission matrices, EEMs) and total synchronous fluorescence spectra (TSFS)were recorded for a series of commercial berry fruit beverages. An analysis of EEMs using parallel factor analysis(PARAFAC)revealed four components characterized by the excitation/ emission maxima at 275/326, 319/410, 414/600, and 360/460 nm, respectively. Based on the spectral profiles, these components were assigned to various groups of phenolic compounds. Partial least squares discriminant analysis was used to develop the classification models. The analysis was performed on PARAFAC scores, unfolded EEMs(uEEMs), unfolded TSFS (uTSFS), and additionally on conventional emission spectra (EMS) measured at particular excitation wavelengths and single synchronous fluorescence spectra (SFS). The classification models with the same average classification error of 4.86% were obtained for the analysis of both the entire uEEMs and uTSFS. Among models based on the individual spectra, the lowest error of 4.42% was obtained for SFS measured at Δλ = 40 nm, and an error of 7.64% was obtained for EMS measured at the excitation wavelength of 360 nm. The classification model based on the PARAFAC scores had the highest error of 15.27%. The present results show good potential of fluorescence as rapid and reagent-free tool for authenticity evaluation of berry beverages.
Author Ewa Sikorska (WT / KTiAI)
Ewa Sikorska,,
- Department of Technology and Instrumental Analysis
, Katarzyna Włodarska (WT / KTiAI)
Katarzyna Włodarska,,
- Department of Technology and Instrumental Analysis
, Igor Khmelinskii - Universidade do Algarve, Portugal
Igor Khmelinskii,,
Journal seriesMethods and Applications in Fluorescence, ISSN 2050-6120, (N/A 100 pkt)
Issue year2020
Publication size in sheets0.6
Keywords in Polishnapoje z owoców jagodowych, widma wzbudzenia-emisji, fluorescencja synchroniczna, PARAFAC, PLS-DA, klasyfikacja
Keywords in EnglishBerry fruit beverages; Excitation-emission matrix; Synchronous fluorescence; PARAFAC; PLS-DA; Classification
ASJC Classification1607 Spectroscopy; 2500 General Materials Science; 3105 Instrumentation; 3107 Atomic and Molecular Physics, and Optics
Languageen angielski
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 19-09-2020, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.496; WoS Impact Factor: 2018 = 2.94 (2) - 2018=3.066 (5)
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