Raspberry seed extract improves the ferroxidase activity of ceruloplasmin in patients with lower artery chronic total occlusion

Bogna Gryszczyńska , Maria Iskra , Maria Małecka , Tomasz Wielkoszyński , Bogdan Pachołek , Anna Gliszczyńska-Świgło , Agnieszka Gryszczyńska , Magdalena Budzyń-Napierała , Magdalena Kasprzak , Krzysztof Strzyżewski

Abstract

Ceruloplasmin (Cp) is the major antioxidant in plasma, a protein that carries about 95% of the total copper. Multiple biochemical activities of ceruloplasmin have been described, including copper transport or oxidation of Fe(II) to Fe(III) for subsequent uptake by transferrin and ferritin. Reduction of the pool of free Fe(II) ions by Cp prevents the generation of free radicals and reactive oxygen species by oxidation of Fe(II), thus inhibiting the Haber-Weiss reaction. The removal of both free Fe(II) and Fe(III) ions from blood plasma by polyphenols enhances the antioxidant system of the living organism. However, the mechanism of interactions between exo- and endogenous antioxidants is still under consideration. The effect of raspberry seed extract (RSE) on the ferroxidase activity of ceruloplasmin isolated from plasma of patients with chronic arterial occlusion of the lower limbs due to atherosclerosis (CpAO) was investigated. Moreover, the effect of RSE on the ferroxidase activity of Cp isolated from healthy volunteers (CpC) was also estimated. The ferroxidase activity of Cp, expressed as ΔFe(II), was determined by spectrophotometry with the use of the Fe(II)–histidine complex and ferrozine as a chromogenic reagent. The addition of RSE to samples with the same amount of both CpAO or CpC in each caused an increase in ΔFe(II). The polyphenol-rich RSE may assist Cp in the fight against free radicals and reactive oxygen species when the disease occurs due to an excessive use or reduced production of endogenous antioxidants.
Author Bogna Gryszczyńska - Uniwersytet Medyczny im. Karola Marcinkowskiego w Poznaniu
Bogna Gryszczyńska,,
-
, Maria Iskra - Poznań University of Medical Sciences
Maria Iskra,,
-
, Maria Małecka (WT / KTAS)
Maria Małecka,,
- Department of Food Commodity Science
, Tomasz Wielkoszyński - Śląska Akademia Medyczna w Zabrzu
Tomasz Wielkoszyński,,
-
, Bogdan Pachołek (WT / KTAS)
Bogdan Pachołek,,
- Department of Food Commodity Science
, Anna Gliszczyńska-Świgło (WT / KTiAI)
Anna Gliszczyńska-Świgło,,
- Department of Technology and Instrumental Analysis
, Agnieszka Gryszczyńska
Agnieszka Gryszczyńska,,
-
, Magdalena Budzyń-Napierała
Magdalena Budzyń-Napierała,,
-
, Magdalena Kasprzak
Magdalena Kasprzak,,
-
, Krzysztof Strzyżewski
Krzysztof Strzyżewski,,
-
Journal seriesJournal of Elementology, ISSN 1644-2296, (A 15 pkt)
Issue year2015
Vol20
No2
Pages305-317
Publication size in sheets0.6
Keywords in Englishraspberry seed extract, atherosclerosis obliterans, ceruloplasmin, ferroxidase activity, natural antioxidants
ASJC Classification1604 Inorganic Chemistry; 2303 Ecology; 2307 Health, Toxicology and Mutagenesis; 2310 Pollution
DOIDOI:10.5601/jelem.2014.19.3.662
URL http://jsite.uwm.edu.pl/index/getfile/662
Languageen angielski
Score (nominal)15
Score sourcejournalList
ScoreMinisterial score = 15.0, 23-12-2019, ArticleFromJournal
Ministerial score (2013-2016) = 15.0, 23-12-2019, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2015 = 0.75; WoS Impact Factor: 2015 = 0.719 (2) - 2015=0.779 (5)
Citation count*
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back
Confirmation
Are you sure?