Evaluation of biologically synthesized Au-CuO and CuO-ZnO nanoparticles against glioma cells and microorganisms

Renata Dobrucka , Mariusz Kaczmarek , Małgorzata Łagiedo , Agata Kielan , Jolanta Długaszewska


Due to the search for new methods of producing bimetallic nanoparticles, in this work, we have conducted a biological synthesis of Au-CuO and CuO-ZnO nanoparticles using Cnici benedicti. The synthesized Au-CuO and CuO-ZnO nanoparticles were also analyzed in terms of their antibacterial activity, as well as their influence on cell viability, using two specific cell lines: C6 rat brain glioma (ATCC® CCL-107™) and T98G human glioma (ATCC® CRL-1690™). The studies carried out by means of Atomic Force Microscopy helped to determine the presence Au-CuO nanoparticles whose size was about 13 nm. The size of CuO-ZnO nanoparticles was about 28 nm. The obtained nanoparticles showed cidal activity against glioma cells depending on the concentration of the substance and the time of culture. In the first stage, the nanoparticles limited the ability to divide cells; then, they blocked the cell cycle in the G2 – M phase, and finally led to massive cell death. The antimicrobial activity studies showed that Au-CuO nanoparticles inhibited the growth of microorganisms at lower concentrations than CuO-ZnO nanoparticles, and both kinds of nanoparticles showed excellent cidal properties.
Author Renata Dobrucka (WT / KTiEPP)
Renata Dobrucka,,
- Department of Industrial Products and Ecology
, Mariusz Kaczmarek - Poznań University of Medical Sciences
Mariusz Kaczmarek,,
, Małgorzata Łagiedo - Uniwersytet Medyczny im. Karola Marcinkowskiego w Poznaniu
Małgorzata Łagiedo,,
, Agata Kielan - Poznań University of Medical Sciences
Agata Kielan,,
, Jolanta Długaszewska - Poznań University of Medical Sciences
Jolanta Długaszewska,,
Journal seriesSaudi Pharmaceutical Journal, ISSN 1319-0164, e-ISSN 2213-7475, (N/A 70 pkt)
Issue year2019
Publication size in sheets0.5
Keywords in Polishnanocząstki metali, aktywność antybakteryjna, działanie przeciwnowotworowe
Keywords in Englishmetal nanoparticles, antimicrobial activity, anticancer activity
ASJC Classification3003 Pharmaceutical Science; 3004 Pharmacology
URL https://www.sciencedirect.com/science/article/pii/S1319016418303803
Languageen angielski
Score (nominal)70
Score sourcejournalList
ScoreMinisterial score = 70.0, 15-04-2020, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 2; WoS Impact Factor: 2018 = 3.643 (2) - 2018=3.675 (5)
Citation count*
Additional fields
First online17 December 2018
Share Share

Get link to the record

* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Are you sure?