Identification of saponins from sugar beet ( Beta vulgaris ) by low and high-resolution HPLC–MS/MS

Katarzyna Mikołajczyk-Bator , Alfred Błaszczyk , Mariusz Czyżniejewski , Piotr Kachlicki


We profiled triterpene saponins from the roots of sugar beet Beta vulgaris L. cultivars Huzar and Boryna using reversed-phase liquid chromatography combined with negative-ion electrospray ionization quadrupole mass spectrometry. We tentatively identified 26 triterpene saponins, including 17 that had not been detected previously in this plant species and 7 saponins that were tentatively identified as new compounds. All observed compounds were glycosides of five different aglycones, of which gypsogenin and norhederagenin are reported for the first time in sugar beet. Thirteen of the saponins detected in sugar beet roots were substituted with dioxolane-type (4 saponins) or acetal-type (9 saponins) dicarboxylic acids. Among the 26 detected saponins, we identified 2 groups of isomers distinguished using high-resolution mass measurements that were detected only in the Huzar cultivar of sugar beet.
Author Katarzyna Mikołajczyk-Bator (WT / KPPJ)
Katarzyna Mikołajczyk-Bator,,
- Department of Natural Science and Quality Assurance
, Alfred Błaszczyk (WT / KPPJ)
Alfred Błaszczyk,,
- Department of Natural Science and Quality Assurance
, Mariusz Czyżniejewski
Mariusz Czyżniejewski,,
, Piotr Kachlicki
Piotr Kachlicki,,
Journal seriesJournal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, ISSN 1570-0232, (A 30 pkt)
Issue year2016
Publication size in sheets0.55
Keywords in Englishsugar beet (Beta vulgaris L.), triterpene saponins, akebonoic acid, oleanolic acid, hederagenin, norhederagenin, HPLC-MS/MS
ASJC Classification2700 General Medicine; 1303 Biochemistry; 1307 Cell Biology; 1308 Clinical Biochemistry; 1602 Analytical Chemistry
Languageen angielski
Score (nominal)30
Score sourcejournalList
ScoreMinisterial score = 30.0, 10-01-2020, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 10-01-2020, ArticleFromJournal
Publication indicators WoS Citations = 6; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.059; WoS Impact Factor: 2016 = 2.603 (2) - 2016=2.711 (5)
Citation count*14 (2021-01-15)
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