Identification of saponins from sugar beet ( Beta vulgaris ) by low and high-resolution HPLC–MS/MS
Katarzyna Mikołajczyk-Bator , Alfred Błaszczyk , Mariusz Czyżniejewski , Piotr Kachlicki
AbstractWe profiled triterpene saponins from the roots of sugar beet Beta vulgaris L. cultivars Huzar and Boryna using reversed-phase liquid chromatography combined with negative-ion electrospray ionization quadrupole mass spectrometry. We tentatively identified 26 triterpene saponins, including 17 that had not been detected previously in this plant species and 7 saponins that were tentatively identified as new compounds. All observed compounds were glycosides of five different aglycones, of which gypsogenin and norhederagenin are reported for the first time in sugar beet. Thirteen of the saponins detected in sugar beet roots were substituted with dioxolane-type (4 saponins) or acetal-type (9 saponins) dicarboxylic acids. Among the 26 detected saponins, we identified 2 groups of isomers distinguished using high-resolution mass measurements that were detected only in the Huzar cultivar of sugar beet.
|Journal series||Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, ISSN 1570-0232, (A 30 pkt)|
|Publication size in sheets||0.55|
|Keywords in English||sugar beet (Beta vulgaris L.), triterpene saponins, akebonoic acid, oleanolic acid, hederagenin, norhederagenin, HPLC-MS/MS|
|ASJC Classification||; ; ; ;|
|Score|| = 30.0, 10-01-2020, ArticleFromJournal|
= 30.0, 10-01-2020, ArticleFromJournal
|Publication indicators||= 6; : 2016 = 1.059; : 2016 = 2.603 (2) - 2016=2.711 (5)|
|Citation count*||11 (2020-08-05)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.