Comparison of Volatiles Profile and Contents of Trichothecenes Group B, Ergosterol, and ATP of Bread Wheat, Durum Wheat, and Triticale Grain Naturally Contaminated by Mycobiota

Maciej Buśko , Kinga Stuper , Henryk Jeleń , Tomasz Góral , Jarosław Chmielewski , Bożena Tyrakowska , Juliusz Perkowski

Abstract

In natural conditions cereals can be infested by pathogenic fungi. These can reduce the grain yield and quality by contamination with mycotoxins which are harmful for plants, animals, and humans. To date, performed studies of the compounds profile have allowed for the distinction of individual species of fungi. The aim of this study was to determine the profile of volatile compounds and trichothecenes of group B, ergosterol, adenosine triphosphate content carried out on a representative sample of 16 genotypes of related cereals: triticale, bread wheat, and durum wheat. Based on an analysis of volatile compounds by means of gas chromatography mass spectrometry and with the use of an electronic nose, volatile profiles for cereals were determined. Differentiation is presented at four levels through discriminant analysis, heatmaps, principal component analysis (PCA), and electronic nose maps. The statistical model was built by subsequent incorporation of chemical groups such as trichothecenes (GC/MS), fungal biomass indicators ergosterol (HPLC) and ATP (luminometric) and volatiles. The results of the discriminatory analyses showed that the volatile metabolites most markedly differentiated grain samples, among which were mainly: lilial, trichodiene, p-xylene. Electronic nose analysis made it possible to completely separate all the analyzed cereals based only on 100 ions from the 50–150 m/z range. The research carried out using chemometric analysis indicated significant differences in the volatile metabolites present in the grain of bread wheat, durum wheat and triticale. The end result of the performed analyses was a complete discrimination of the examined cereals based on the metabolites present in their grain.
Author Maciej Buśko - Uniwersytet Przyrodniczy w Poznaniu
Maciej Buśko,,
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, Kinga Stuper - Uniwersytet Przyrodniczy w Poznaniu
Kinga Stuper,,
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, Henryk Jeleń - Uniwersytet Przyrodniczy w Poznaniu, MNiSW [80]
Henryk Jeleń,,
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, Tomasz Góral - Instytut Hodowli i Aklimatyzacji Roślin – Państwowy Instytut Badawczy
Tomasz Góral,,
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, Jarosław Chmielewski (WT / KTiAI)
Jarosław Chmielewski,,
- Department of Technology and Instrumental Analysis
, Bożena Tyrakowska (WT / KTiAI)
Bożena Tyrakowska,,
- Department of Technology and Instrumental Analysis
, Juliusz Perkowski - Uniwersytet Przyrodniczy w Poznaniu
Juliusz Perkowski,,
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Journal seriesFrontiers in Plant Science, ISSN 1664-462X, (A 40 pkt)
Issue year2016
No7
Pages1-16
Publication size in sheets0.75
Keywords in Polishlotne związki organiczne, trichoteceny, ergosterol, ATP, pszenica, pszenżyto
Keywords in Englishvolatile organic compounds, trichothecenes, ergosterol, ATP, bread wheat, durum wheat, triticale
ASJC Classification1110 Plant Science
DOIDOI:10.3389/fpls.2016.01243
URL http://journal.frontiersin.org/article/10.3389/fpls.2016.01243/full
Languageen angielski
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 40.0, 05-03-2020, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 05-03-2020, ArticleFromJournal
Publication indicators WoS Citations = 7; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.241; WoS Impact Factor: 2016 = 4.298 (2) - 2016=4.677 (5)
Citation count*13 (2020-09-12)
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