The system with zwitterionic lactose-based surfactant for complexation and delivery of small interfering ribonucleic acid—A structural and spectroscopic study

Michalina Skupin , Krzysztof Sobczak , Ryszard Zieliński , Maciej Kozak

Abstract

Systems suitable for the effective preparation of complexes with siRNA (small interfering RNA) are at the center of interest in the area of research work on the delivery of the RNA-based drugs (RNA-therapeutics). This article presents results of a study on the structural effects associated with siRNA complexation by a surfactant comprising a lactose group (N-(3-propanesulfone)-N-dodecyl-amino-beta-D-lactose hydrochloride, LA12). The double stranded siRNA oligomer (21 base pairs) used in this study is responsible for silencing a gene that can be important in the therapy of myotonic dystrophy type 1. The obtained siRNA/LA12 lipoplexes were studied using the methods of small angle scattering of synchrotron radiation, circular dichroism spectroscopy, Fourier transform infrared spectroscopy, and electrophoretic mobility tests. Lipoplexes form in solution stable lamellar or cubic phases. The surfactant selected for the study shows much lower cytotoxicity and good complexation abilities of siRNA than dicationic or polycationic surfactants.
Author Michalina Skupin - Adam Mickiewicz University (UAM)
Michalina Skupin,,
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, Krzysztof Sobczak - University Adam Mickiewicz in Poznań
Krzysztof Sobczak,,
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, Ryszard Zieliński (WT / KTiAI)
Ryszard Zieliński,,
- Department of Technology and Instrumental Analysis
, Maciej Kozak - University Adam Mickiewicz in Poznań
Maciej Kozak,,
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Journal seriesApplied Physics Letters, ISSN 0003-6951, (A 40 pkt)
Issue year2016
Vol108
No21
Pages1-5
Publication size in sheets0.5
Keywords in Polishsurfaktanty, sulfobetainy, si-RNA, oddziaływania międzycząsteczkowe
ASJC Classification3101 Physics and Astronomy (miscellaneous)
DOIDOI:10.1063/1.4952589
URL https://aip.scitation.org/doi/10.1063/1.4952589
Languageen angielski
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 35.0, 05-02-2020, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 05-02-2020, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.266; WoS Impact Factor: 2016 = 3.411 (2) - 2016=3.341 (5)
Citation count*1 (2020-09-23)
Additional fields
UwagiNumeracja stron w artykule: 213701-1 - 213701-5
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