The system with zwitterionic lactose-based surfactant for complexation and delivery of small interfering ribonucleic acid—A structural and spectroscopic study
Michalina Skupin , Krzysztof Sobczak , Ryszard Zieliński , Maciej Kozak
AbstractSystems suitable for the effective preparation of complexes with siRNA (small interfering RNA) are at the center of interest in the area of research work on the delivery of the RNA-based drugs (RNA-therapeutics). This article presents results of a study on the structural effects associated with siRNA complexation by a surfactant comprising a lactose group (N-(3-propanesulfone)-N-dodecyl-amino-beta-D-lactose hydrochloride, LA12). The double stranded siRNA oligomer (21 base pairs) used in this study is responsible for silencing a gene that can be important in the therapy of myotonic dystrophy type 1. The obtained siRNA/LA12 lipoplexes were studied using the methods of small angle scattering of synchrotron radiation, circular dichroism spectroscopy, Fourier transform infrared spectroscopy, and electrophoretic mobility tests. Lipoplexes form in solution stable lamellar or cubic phases. The surfactant selected for the study shows much lower cytotoxicity and good complexation abilities of siRNA than dicationic or polycationic surfactants.
|Journal series||Applied Physics Letters, ISSN 0003-6951, (A 40 pkt)|
|Publication size in sheets||0.5|
|Keywords in Polish||surfaktanty, sulfobetainy, si-RNA, oddziaływania międzycząsteczkowe|
|Score|| = 35.0, 05-02-2020, ArticleFromJournal|
= 40.0, 05-02-2020, ArticleFromJournal
|Publication indicators||: 2016 = 1.266; : 2016 = 3.411 (2) - 2016=3.341 (5)|
|Citation count*||1 (2020-08-04)|
|Uwagi||Numeracja stron w artykule: 213701-1 - 213701-5|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.