The impact of polyphenols on Bifidobacterium growth

Daniela Gwiazdowska , Krzysztof Juś , Joanna Jasnowska-Małecka , Katarzyna Kluczyńska

Abstract

Polyphenols are a common group of plant based bioactive compounds, that can affect human health because of their antioxidant and antimicrobial properties as well as free-radical scavenging activity. An increasing interest is observed in the interaction between polyphenols and microbiota occurring in food and the human gut. The aim of the work presented here, was to evaluate the effect of some polyphenolic compounds on the growth of two strains of Bifidobacterium: B. adolescentis and B. bifidum. The influence of some flavonoids: naringinin, hesperidin, rutin, quercetin as well as phenolic acids: gallic, caffeic, p-coumaric, ferulic, chlorogenic, vanillic and sinapic was determined by a 96-well microtiter plate assay. In the experiments the effect of three different concentrations of polyphenols: 2, 20 and 100 µg/ml on the growth of Bifidobacterium strains was investigated. All tested compounds influenced the growth of the examined bacteria. Both stimulatory and inhibitory effects were observed in comparison to the positive control. The strongest impact on the growth of bifidobacteria was observed during the first hours of incubation. The constant inhibitory effect was observed for hesperidin and quercetin addition and was dose-dependent. B. bifidum showed a stronger dependence on phenolic acids content in the medium than B. adolescentis during the first hours of incubation.
Author Daniela Gwiazdowska (WT / KPPJ)
Daniela Gwiazdowska,,
- Department of Natural Science and Quality Assurance
, Krzysztof Juś (WT / KPPJ)
Krzysztof Juś,,
- Department of Natural Science and Quality Assurance
, Joanna Jasnowska-Małecka (WT / KPPJ)
Joanna Jasnowska-Małecka,,
- Department of Natural Science and Quality Assurance
, Katarzyna Kluczyńska (WT / KPPJ)
Katarzyna Kluczyńska,,
- Department of Natural Science and Quality Assurance
Journal seriesActa Biochimica Polonica, ISSN 0001-527X, (A 15 pkt)
Issue year2015
Vol62
No4
Pages895-901
Publication size in sheets0.5
Keywords in Englishdietary polyphenol, probiotic, inhibition, stimulation
ASJC Classification1300 General Biochemistry, Genetics and Molecular Biology
DOIDOI:10.18388/abp.2015_1154
URL http://www.actabp.pl/pdf/4_2015/2015_1154.pdf
Languageen angielski
Score (nominal)15
Score sourcejournalList
ScoreMinisterial score = 15.0, 23-12-2019, ArticleFromJournal
Ministerial score (2013-2016) = 15.0, 23-12-2019, ArticleFromJournal
Publication indicators WoS Citations = 5; Scopus SNIP (Source Normalised Impact per Paper): 2015 = 0.74; WoS Impact Factor: 2015 = 1.187 (2) - 2015=1.534 (5)
Citation count*35 (2020-08-07)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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